ZFIN ID: ZDB-FIG-080408-1
Kedde et al., 2007 - RNA-Binding Protein Dnd1 Inhibits MicroRNA Access to Target mRNA. Cell   131(7):1273-1286 Full text @ Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: 1-cell to Adult

Fig. 3 Zebrafish Dnd1 (drDnd1) Counteracts Inhibition of Nanos by miR-430
(A) One-cell-stage zebrafish embryos were injected with Dnd1 morpholino or control morpholino. RNA was extracted and subjected to quantitative RT-PCR analysis to compare endogenous levels of nanos1 and vasa to odc, and nanos to vasa.
(B) One-cell-stage zebrafish embryos were coinjected with DsRed-nos1-3′UTR and gfp-vasa-3′UTR together with dead end morpholino or control morpholino.
(C) One-cell-stage zebrafish embryos were coinjected with RNA containing the venus open-reading frame fused to the wild-type nanos1 3′UTR (3′nos1wt), RNA containing the cfp open-reading frame fused to the miR-430-binding site mutated nanos1 3′UTR (cfp-3′nos1mut1) and vasa-dsRed (for labeling the germinal granule for easier identification of germ cells) together with dead end morpholino or control morpholino. Error bars depict the standard error of the mean (SEM); the p value was calculated using t test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ddx4 WT control 1-cell to Adult whole organism RTPCR
WT + MO1-dnd1 standard conditions 1-cell to Adult whole organism RTPCR
nanos3 WT control 1-cell to Adult whole organism RTPCR
WT + MO1-dnd1 standard conditions 1-cell to Adult whole organism RTPCR
odc1 WT control 1-cell to Adult whole organism RTPCR
WT + MO1-dnd1 standard conditions 1-cell to Adult whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Cell, 131(7), Kedde, M., Strasser, M.J., Boldajipour, B., Oude Vrielink, J.A., Slanchev, K., le Sage, C., Nagel, R., Voorhoeve, P.M., van Duijse, J., Andersson ├śrom, U., Lund, A.H., Perrakis, A., Raz, E., and Agami, R., RNA-Binding Protein Dnd1 Inhibits MicroRNA Access to Target mRNA, 1273-1286, Copyright (2007) with permission from Elsevier. Full text @ Cell