ZFIN ID: ZDB-IMAGE-080701-8
Figures for Kedde et al., 2007

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Figure Caption/Comments:

Fig. 3 Zebrafish Dnd1 (drDnd1) Counteracts Inhibition of Nanos by miR-430
(A) One-cell-stage zebrafish embryos were injected with Dnd1 morpholino or control morpholino. RNA was extracted and subjected to quantitative RT-PCR analysis to compare endogenous levels of nanos1 and vasa to odc, and nanos to vasa.
(B) One-cell-stage zebrafish embryos were coinjected with DsRed-nos1-3′UTR and gfp-vasa-3′UTR together with dead end morpholino or control morpholino.
(C) One-cell-stage zebrafish embryos were coinjected with RNA containing the venus open-reading frame fused to the wild-type nanos1 3′UTR (3′nos1wt), RNA containing the cfp open-reading frame fused to the miR-430-binding site mutated nanos1 3′UTR (cfp-3′nos1mut1) and vasa-dsRed (for labeling the germinal granule for easier identification of germ cells) together with dead end morpholino or control morpholino. Error bars depict the standard error of the mean (SEM); the p value was calculated using t test.

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