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ZFIN ID: ZDB-FIG-080306-3
Sarmah et al., 2007 - A role for the inositol kinase Ipk1 in ciliary beating and length maintenance. Proceedings of the National Academy of Sciences of the United States of America   104(50):19843-19848 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. S7

Codepletion of Ipk1 and either IFT88 or IFT57 synergistically perturbs LR asymmetry. Embryos obtained from crossing wild-type fish were injected with varying amounts of either single MO (ipk1MO1, ift88MO, ift57MO, control MO) or a MO combination (ipk1MO1 + ift88MO, ipk1MO1 + ift57MO, control MO + ift88MO, control MO + ift57MO). On the other hand, embryos derived from crossing heterozygous ift88 (previously known as polaris or oval) mutant (ift88+/-) fish were injected with varying amounts of either ipk1MO1 or control MO. Scores on the heart-tube placement defect in embryos at 28 hpf from each treatment are presented as bar graphs.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ift57 standard conditions Prim-5 heart tube position, abnormal
WT + MO1-ift57 + MO1-ippk standard conditions Prim-5 heart tube position, abnormal
WT + MO1-ift88 + MO1-ippk standard conditions Prim-5 heart tube position, abnormal
WT + MO1-ippk standard conditions Prim-5 heart tube position, abnormal
ift88tz288/+ + MO1-ippk standard conditions Prim-5 heart tube position, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA