PUBLICATION

A role for the inositol kinase Ipk1 in ciliary beating and length maintenance

Authors
Sarmah, B., Winfrey, V.P., Olson, G.E., Appel, B., and Wente, S.R.
ID
ZDB-PUB-071210-31
Date
2007
Source
Proceedings of the National Academy of Sciences of the United States of America   104(50): 19843-19848 (Journal)
Registered Authors
Appel, Bruce
Keywords
cilia, inositol hexakisphosphate, kidney development, left–right asymmetry, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Biological Transport
  • Body Patterning
  • Cell Line
  • Centrosome/metabolism
  • Cilia/enzymology*
  • Cilia/physiology*
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Humans
  • Microtubules/metabolism
  • Phosphotransferases (Alcohol Group Acceptor)/genetics
  • Phosphotransferases (Alcohol Group Acceptor)/metabolism*
  • Protozoan Proteins/genetics
  • Protozoan Proteins/metabolism
  • Zebrafish*/embryology
  • Zebrafish*/genetics
  • Zebrafish*/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
18056639 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
Cilia project from cells as membranous extensions, with microtubule structural cores assembling from basal bodies by intraflagellar transport (IFT). Here, we report a ciliary role for the inositol 1,3,4,5,6-pentakisphosphate 2-kinase (Ipk1) that generates inositol hexakisphosphate. In zebrafish embryos, reducing Ipk1 levels inhibited ciliary beating in Kupffer's vesicle and decreased ciliary length in the spinal canal, pronephric ducts, and Kupffer's vesicle. Electron microscopy showed that ciliary axonemal structures were not grossly altered. However, coincident knockdown of Ipk1 and IFT88 or IFT57 had synergistic perturbations. With GFP-Ipk1 enriched in centrosomes and basal bodies, we propose that Ipk1 plays a previously uncharacterized role in ciliary function.
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Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes