FIGURE

Fig. 3

ID
ZDB-FIG-080301-3
Publication
Sarmah et al., 2007 - A role for the inositol kinase Ipk1 in ciliary beating and length maintenance
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Fig. 3

Ipk1 links to IFT and microtubule-mediated organelle transport. (A and B) Codepletion of Ipk1 and either IFT88 or IFT57 synergistically perturbs LR asymmetry. (A) Embryos were injected with varying amounts of either a single MO (ipk1 MO1, ift88 MO, ift57 MO, control MO) or a MO combination (ipk1 MO1+ift88 MO, ipk1 MO1+ift57 MO, control MO+ift88 MO, control MO+ift57 MO). (B) Embryos derived from crossing either heterozygous ift88 +/- mutant or wild-type fish were injected with ipk1 MO1 or control MO. Scores for heart-tube placement defect in embryos at 28 hpf are graphed. (C and D) ipk1 knockdown perturbs microtubule-mediated organelle transport. (C) Five days after fertilization embryos [uninjected and ipk1 MO1-injected (Left)] were treated with 0.5 mg/ml epinephrine, and time (min) required for all melanosomes in the head and trunk (within the white rectangle) to become perinuclear (Center) was determined. Time required for retracted melanosomes in epinephrine-treated endpoint embryos to fully disperse on exposure to 1 mg/ml caffeine (Right) was also determined. (D) Graph of the response time for epinephrine and caffeine treatments in uninjected and ipk1 MO1 embryos.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Day 5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA