ZFIN ID: ZDB-FIG-080301-3
Sarmah et al., 2007 - A role for the inositol kinase Ipk1 in ciliary beating and length maintenance. Proceedings of the National Academy of Sciences of the United States of America   104(50):19843-19848 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Day 5

Fig. 3

Ipk1 links to IFT and microtubule-mediated organelle transport. (A and B) Codepletion of Ipk1 and either IFT88 or IFT57 synergistically perturbs LR asymmetry. (A) Embryos were injected with varying amounts of either a single MO (ipk1 MO1, ift88 MO, ift57 MO, control MO) or a MO combination (ipk1 MO1+ift88 MO, ipk1 MO1+ift57 MO, control MO+ift88 MO, control MO+ift57 MO). (B) Embryos derived from crossing either heterozygous ift88 +/- mutant or wild-type fish were injected with ipk1 MO1 or control MO. Scores for heart-tube placement defect in embryos at 28 hpf are graphed. (C and D) ipk1 knockdown perturbs microtubule-mediated organelle transport. (C) Five days after fertilization embryos [uninjected and ipk1 MO1-injected (Left)] were treated with 0.5 mg/ml epinephrine, and time (min) required for all melanosomes in the head and trunk (within the white rectangle) to become perinuclear (Center) was determined. Time required for retracted melanosomes in epinephrine-treated endpoint embryos to fully disperse on exposure to 1 mg/ml caffeine (Right) was also determined. (D) Graph of the response time for epinephrine and caffeine treatments in uninjected and ipk1 MO1 embryos.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: adrenaline Day 5 pigment granule dispersal rate, normal
WT chemical treatment: (R)-adrenaline Day 5 pigment granule aggregation in cell center decreased rate, abnormal
WT + MO1-ift57 + MO1-ippk standard conditions Prim-5 heart tube position, abnormal
WT + MO1-ift88 + MO1-ippk standard conditions Prim-5 heart tube position, abnormal
ift88tz288/+ + MO1-ippk standard conditions Prim-5 heart tube position, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA