ZFIN ID: ZDB-FIG-080301-1
Sarmah et al., 2007 - A role for the inositol kinase Ipk1 in ciliary beating and length maintenance. Proceedings of the National Academy of Sciences of the United States of America   104(50):19843-19848 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 5-9 somites to Prim-15

Fig. 1

ipk1 knockdown reduces normal ciliary beating and length. (A and B) Schematic diagrams (A) and kymographs (B) showing trajectory of a cilium in uninjected, ipk1 MO1-injected, ipk1 MO1 plus wild-type ipk1 mRNA-injected, or ipk1 MO1 plus kinase-dead (kd) ipk1 mRNA-injected embryos. (C) Graphs for measurements of ciliary length from individual embryos (green), with the mean ciliary length (far right, purple) determined from multiple independent measurements for KV (n > 26), pronephric duct (n > 13), and spinal canal (n > 14) cilia. (D) Representative images of KV cilia (red) from embryos injected with 10 ng of either control MO or ipk1 MO1. n, number of cilia measured per embryo.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ippk standard conditions 5-9 somites Kupffer's vesicle cilium decreased functionality, abnormal
5-9 somites Kupffer's vesicle cilium decreased length, abnormal
Prim-15 central canal cilium decreased functionality, abnormal
Prim-15 central canal cilium decreased length, abnormal
Prim-15 pronephric duct cilium decreased functionality, abnormal
Prim-15 pronephric duct cilium decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA