ZFIN ID: ZDB-FIG-070620-45
Hans et al., 2007 - Changes in retinoic acid signaling alter otic patterning. Development (Cambridge, England)   134(13):2449-2458 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: 26+ somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 26+ somites to Long-pec

Fig. 7 Rhombomere 5-specific Wnt8b function is required for the maintenance of otic fate. (A-D) Assessed by morphology, otic vesicles are reduced in fgf3 (C) and tcf2 (D) single mutants compared to wild-type embryos (A), but not as much as in RA-depleted embryos (B). (E) Loss of both fgf3 and tcf2 together results in otic vesicles that form only one otolith, as is observed in RA-depleted embryos (B). (F,G) Expression of dlx3b in the dorsal half of the otocyst at 22 hpf is lost in embryos depleted of RA signaling. (H-J) In control embryos at 22 hpf, wnt8b expression can be detected in the midbrain-hindbrain boundary, in r3 and in r5 (H), whereas tcf2 mutants (I) and RA-depleted embryos (J) show wnt8b in the midbrain-hindbrain boundary and in r3, but not in r5. (K-N) Inactivation of Wnt8b in wild-type embryos by morpholino injection (MO, N) leads to a reduced ear size (labeled with stm at 22 hpf), similar to the size observed in fgf3 mutants (M), compared to untreated wild type (K); however, the size reduction is not as severe as in embryos depleted of RA signaling (L). Loss of Wnt8b function in fgf3 mutants (O) produces reduced otic vesicles comparable to RA-depleted embryos (L). (A-G) Lateral views with anterior to the left and dorsal towards the top. (H-O) Dorsal views with anterior towards the top. Scale bar: 35 µm for A-E and H-O; 20 µm for F,G.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx3b AB control 26+ somites otic vesicle ISH
AB chemical treatment by environment: 4-(diethylamino)benzaldehyde 26+ somites Not Detected otic vesicle ISH
stm AB control 26+ somites otic vesicle ISH
AB chemical treatment by environment: 4-(diethylamino)benzaldehyde 26+ somites otic vesicle ISH
AB + MO1-wnt8b standard conditions 26+ somites otic vesicle ISH
fgf3t24149/t24149 control 26+ somites otic vesicle ISH
fgf3t24149/t24149 + MO1-wnt8b standard conditions 26+ somites otic vesicle ISH
wnt8b AB control 26+ somites midbrain hindbrain boundary ISH
26+ somites rhombomere 3 ISH
26+ somites rhombomere 5 ISH
AB chemical treatment by environment: 4-(diethylamino)benzaldehyde 26+ somites midbrain hindbrain boundary ISH
26+ somites rhombomere 3 ISH
26+ somites Not Detected rhombomere 5 ISH
hnf1bahi2169Tg/hi2169Tg control 26+ somites midbrain hindbrain boundary ISH
26+ somites rhombomere 3 ISH
26+ somites Not Detected rhombomere 5 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-wnt8b standard conditions 26+ somites otic vesicle decreased size, abnormal
fgf3t24149/t24149 control 26+ somites otic vesicle decreased size, abnormal
Long-pec otic vesicle decreased size, abnormal
fgf3t24149/t24149 + MO1-wnt8b standard conditions 26+ somites otic vesicle decreased size, abnormal
fgf3t24149/t24149; hnf1bahi2169Tg/hi2169Tg control Long-pec otic vesicle decreased size, abnormal
Long-pec otolith organ decreased amount, abnormal
hnf1bahi2169Tg/hi2169Tg control 26+ somites hindbrain morphogenesis disrupted, abnormal
Long-pec otic vesicle decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development