ZFIN ID: ZDB-FIG-070620-44
Hans et al., 2007 - Changes in retinoic acid signaling alter otic patterning. Development (Cambridge, England)   134(13):2449-2458 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 5-9 somites to 26+ somites
PHENOTYPE:
Fish:
Observed In:
Stage: 26+ somites

Fig. 6 Misexpression of fgf8 at the end of gastrulation rescues otic induction in RA-depleted embryos, but the rescue is not maintained. (A,B) Preotic pax8 expression is expanded in transgenic hsp:fgf8 embryos (B) after misexpression of fgf8 at the end of gastrulation in comparison to non-transgenic embryos (A). (C,D) Reduced preotic pax8 expression in RA-depleted embryos (C) can be rescued by the misexpression of fgf8 at the end of gastrulation (D), as can heat-shocked transgenic hsp:fgf8 embryos with normal RA signaling (B). (E,F). Misexpression of fgf8 at late gastrulation stages (F) leads to the formation of much larger otic vesicles in comparison to their non-transgenic siblings (E). (G,H) Transgenic hsp:fgf8 embryos depleted of RA signaling (H) do not show an increase in otic cells but rather resemble non-transgenic RA-depleted siblings (G). Dorsal views with anterior towards the top. Scale bar: 35 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pax8 AB control 5-9 somites otic placode ISH
AB chemical treatment by environment: 4-(diethylamino)benzaldehyde 5-9 somites otic placode ISH
b1193Tg (AB) control 5-9 somites otic placode ISH
b1193Tg (AB) chemical treatment by environment: 4-(diethylamino)benzaldehyde 5-9 somites otic placode ISH
stm AB control 26+ somites otic vesicle ISH
AB chemical treatment by environment: 4-(diethylamino)benzaldehyde 26+ somites otic vesicle ISH
b1193Tg (AB) control 26+ somites otic vesicle ISH
b1193Tg (AB) chemical treatment by environment: 4-(diethylamino)benzaldehyde 26+ somites otic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
b1193Tg (AB) control 26+ somites otic vesicle increased size, abnormal
Acknowledgments:
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