ZFIN ID: ZDB-FIG-070620-42
Hans et al., 2007 - Changes in retinoic acid signaling alter otic patterning. Development (Cambridge, England)   134(13):2449-2458 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-5

Fig. 4 Increased RA signaling compromises lens specification. (A-F) Assessed both by morphology at 50 hpf (DIC microscopy, A-C) and cx44.1 expression at 24 hpf (D-F), the lens is reduced in size or completely lost after the application of 10 or 20 nM RA, compared with wild-type embryos. (G-I) Compromised lens specification is already evident at preplacodal stages (five-somite stage, 5S), as indicated by pax6b labeling. (A-C) Lateral views of live eyes with anterior to the left and dorsal towards the top. (D-I) Dorsal views with anterior towards the top. Scale bar: 30 µm for A-C; 50 µm for D-F; 35 µm for G-I.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gja8b AB control Prim-5 solid lens vesicle ISH
AB chemical treatment: all-trans-retinoic acid Prim-5 solid lens vesicle ISH
AB chemical treatment: all-trans-retinoic acid Prim-5 Not Detected solid lens vesicle ISH
pax6b AB control 5-9 somites optic vesicle ISH
AB chemical treatment: all-trans-retinoic acid 5-9 somites optic vesicle ISH
AB chemical treatment: all-trans-retinoic acid 5-9 somites optic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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