ZFIN ID: ZDB-FIG-070314-60
Huang et al., 2007 - Amotl2 is essential for cell movements in zebrafish embryo and regulates c-Src translocation. Development (Cambridge, England)   134(5):979-988 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: 1-4 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 75%-epiboly to 5-9 somites

Fig. 4 Effects of amotl2 expression knockdown on embryonic cell movements. (A) Injection of amotl2 morpholino led to slower epiboly and impaired CE movement. Single-cell embryos were injected with amotl2MO1 (MO1), amotl2MO2 (MO2) or control morpholino (cMO1) at the indicated doses. The same embryo was pictured every 2 hours and presented here. Embryos are shown as lateral views with animal pole to the top and dorsal to the right. Arrows denote the anterior tip of the hypoblast, and arrowheads point to the tail region. (B) Injection of amotl2 morpholino affected convergent extension. Injected embryos at the two-somite stage were fixed and simultaneously hybridized to hgg1 (red), dlx3 (blue) and ntl (blue) probes. Each embryo was placed in different orientation: in the top panel, anterodorsal views show relative positions of the hgg1 and dlx3 domains; in the middle panel, dorsal views show the ntl domain; and in the bottom panel, lateral views with dorsal to the right show the length of the ntl domain and the embryonic anteroposterior axis. (C) Effect of amotl2 morpholino injection was alleviated by overexpression of modified amotl2 (amotl2m) mRNA. Groups of live embryos were pictured at the indicated stages.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb TU control 1-4 somites unspecified ISH
TU + MO1-amotl2a standard conditions 1-4 somites unspecified ISH
dlx3b TU control 1-4 somites unspecified ISH
TU + MO1-amotl2a standard conditions 1-4 somites unspecified ISH
tbxta TU control 1-4 somites unspecified ISH
TU + MO1-amotl2a standard conditions 1-4 somites unspecified ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-amotl2a standard conditions 75%-epiboly - 5-9 somites convergent extension decreased rate, abnormal
1-4 somites embryonic pattern specification disrupted, abnormal
1-4 somites neural plate increased width, abnormal
1-4 somites polster mislocalised, abnormal
1-4 somites whole organism wholly dorsalized, abnormal
5-9 somites whole organism decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development