ZFIN ID: ZDB-FIG-070214-3
Zecchin et al., 2007 - Distinct delta and jagged genes control sequential segregation of pancreatic cell types from precursor pools in zebrafish. Developmental Biology   301(1):192-204 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Day 4

Fig. 6  Effects of morpholino-mediated knock down of Notch ligands on the differentiation of pancreatic cell populations. Panels A–D: insulin and glucagon expression at 24 hpf in control and deltaA-morphant embryos. glucagon-expressing cells are decreased in number in deltaA morphants (D). Panels E–H: glucagon expression in the pancreas (E, F) and gut (G, H) is increased in jagged1b-morphant embryos (F, H) compared with controls (E, G). Panels I–J: trypsin expression is decreased in jagged1b morphant embryos. Panels K–N: expression of endocrine and exocrine markers in jagged2-morphant embryos. insulin (red) expression is increased and trypsin (blue) expression decreased in jagged2-morphants (panels L and L′ blow up) compared with controls (panels K and K′ blow up). glucagon expression profile in jagged2-morphants is enlarged (N) reflecting the increased size of the beta-cell cluster. Embryos are in ventral view with anterior to the left. Stages are indicated at the bottom right of each frame. The scale bar is 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gcga WT + MO1-dla standard conditions Prim-5 pancreas primordium ISH
WT + MO2-jag1b standard conditions Long-pec pancreas ISH
Protruding-mouth gut ISH
WT + MO2-jag2b standard conditions Protruding-mouth pancreas ISH
ins WT + MO1-dla standard conditions Prim-5 pancreas primordium ISH
WT + MO2-jag2b standard conditions Protruding-mouth pancreas ISH
prss1 WT + MO2-jag1b standard conditions Day 4 pancreas ISH
WT + MO2-jag2b standard conditions Protruding-mouth pancreas ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 301(1), Zecchin, E., Filippi, A., Biemar, F., Tiso, N., Pauls, S., Ellertsdottir, E., Gnugge, L., Bortolussi, M., Driever, W., and Argenton, F., Distinct delta and jagged genes control sequential segregation of pancreatic cell types from precursor pools in zebrafish, 192-204, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.