ZFIN ID: ZDB-FIG-060901-16
Yang et al., 2006 - Small molecule-induced ablation and subsequent regeneration of larval zebrafish melanocytes. Development (Cambridge, England)   133(18):3563-3573 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Prim-5

Fig. 2 Melanocyte lineages develop to late stages in the presence of MoTP. (A-H) The development of melanocyte lineages in MoTP-treated (B,D,F,H) and untreated (A,C,E,G) larvae were examined by whole-mount RNA in situ hybridization with mitf (A,B), kit (C,D), tyr (E,F) and dct (G,H). For these experiments, embryos were incubated in MoTP solution from 14 to 27 hpf, then immediately fixed for in situ analysis. The developmental patterns and the numbers of cells (white arrowheads) for each of the markers are indistinguishable in the MoTP-treated larvae from those in untreated larvae. Untreated larvae were reared in PTU to completely block melanin synthesis, thereby allowing easier visualization of NBT/BCIP precipitates in melanocytes. Scale bar: 500 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dct WT control Prim-5 melanoblast ISH
WT chemical treatment: pharmaceutical Prim-5 melanoblast ISH
kita WT control Prim-5 melanoblast ISH
WT chemical treatment: pharmaceutical Prim-5 melanoblast ISH
mitfa WT control Prim-5 melanoblast ISH
WT chemical treatment: pharmaceutical Prim-5 melanoblast ISH
tyr WT control Prim-5 melanoblast ISH
WT chemical treatment: pharmaceutical Prim-5 melanoblast ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development