FIGURE

Fig. 5

ID

ZDB-FIG-050810-874

Publication

Chatterjee et al., 2005 - Developmental regulation and expression of the zebrafish connexin43 gene

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Fig. 5

Temporal expression of Cx43α transcript and protein. A: Autoradiography of RNase protection analysis of Cx43α mRNA through 120 hr of development. eF-1mRNA was used as a loading control. B: Quantitative analysis of the Cx43α mRNA levels determined by RNase protection. Note that a standard curve was generated using synthetic Cx43α transcripts, with the specific levels of embryonic Cx43α mRNA in picograms being reported on the Y axis. C: Western immunoblot analysis revealing the presence of Cx43α protein in 48 hpf embryos and adult heart and ovary. The mobility of the Cx43α band in the zebrafish samples was not altered by alkaline phosphatase treatment. Lane 1, 48 hours postfertilization (hpf) embryo extract treated with phosphatase inhibitors; lane 2, 48 hpf embryo extract treated with CIP; lane 3, adult zebrafish heart extract treated with phosphatase inhibitors; lane 4, adult zebrafish heart extract treated with CIP; lane 5, adult mouse ovary extract treated with phosphatase inhibitors; lane 6, adult mouse ovary extract treated with CIP; lane 7, adult zebrafish ovary extract treated with phosphatase inhibitors; lane 8, adult zebrafish ovary extract treated with CIP.

Expression Data

Gene:	gja1b
Fish:	WT
Anatomical Terms:	ovary whole organism
Stage Range:	1-cell to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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