ZFIN ID: ZDB-FIG-050715-9
Rhinn et al., 2005 - Positioning of the midbrain-hindbrain boundary organizer through global posteriorization of the neuroectoderm mediated by Wnt8 signaling. Development (Cambridge, England)   132(6):1261-1272 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Shield to 75%-epiboly

Fig. 5 gbx1 and otx2 expression in Wnt8 loss-of-function experiments. (A-C) Embryos stained for gbx1 and (D-F) embryos stained for gbx1 and Ntl protein (brown). (A-C) Control embryo (A), embryo injected with MoWnt8 (B) and Df(Wnt8) mutant embryos (C) at 60%. gbx1 is not expressed at 60% in absence of Wnt8. (D-F) Control embryo (D), embryo injected with MoWnt8 (E) and Df(Wnt8) mutant embryo (F) at 80%. gbx1 expression is observed at 80% onwards and overlaps partially with the Ntl domain. (G-L) Embryos stained for otx2 and Ntl protein (brown). (G-I) Control embryos (G), embryo injected with MoWnt8 (H) and Df(Wnt8) mutant embryo (I) at 60%. (J-L) Control embryos (J), embryos injected with MoWnt8 (K) and Df(Wnt8) mutant embryo (L) at 80%. Laterally a posterior shift of the otx2 expression domain is visible (red arrowheads) in the morphants and in the Df(Wnt8) mutant embryos. (A-L) Dorsal views, anterior upwards.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gbx1 Df(Chr14:wnt8a)w8/w8 standard conditions Shield Not Detected blastoderm ISH
75%-epiboly blastoderm ISH
75%-epiboly margin ISH
WT standard conditions Shield blastoderm ISH
75%-epiboly blastoderm ISH
WT + MO1-wnt8a standard conditions Shield Not Detected blastoderm ISH
75%-epiboly blastoderm ISH
75%-epiboly margin ISH
otx2b Df(Chr14:wnt8a)w8/w8 standard conditions Shield blastoderm ISH
75%-epiboly blastoderm ISH
WT standard conditions Shield blastoderm ISH
75%-epiboly blastoderm ISH
WT + MO1-wnt8a standard conditions Shield blastoderm ISH
75%-epiboly blastoderm ISH
tbxta Df(Chr14:wnt8a)w8/w8 standard conditions Shield margin IHC
75%-epiboly margin IHC
WT standard conditions Shield margin IHC
75%-epiboly margin IHC
WT + MO1-wnt8a standard conditions Shield margin IHC
75%-epiboly margin IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development