ZFIN ID: ZDB-FIG-050221-6
Wilm et al., 2005 - Essential roles of a zebrafish prdm1/blimp1 homolog in embryo patterning and organogenesis. Development (Cambridge, England)   132(2):393-404 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Bud
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 5-9 somites to Prim-5

Fig. 7 Early loss-of-function phenotype after MO-mediated interference with Prdm1 activity. (A-E) Ventral view, anterior to the left; tail region is shown. (F-I,N-Q,U-X) Lateral view, dorsal to the right. (J-M) Animal view, dorsal to the bottom. (R,S) Dorsal view, anterior to the top; somitic region is shown. (F,J;G,K;H,L;I,M) Different views of same embryo. (A,B,D,F,H,J,L,N,P,R,U,W) Untreated controls (ctrl). (C,E) Embryos microinjected with 1 ng of MOprdm1 (MO). (G,I,K,M,O,Q,S,V,X) Embryos microinjected with 2 ng of MOprdm1. (A) Wild-type tail. (B,C) Interference with Prdm1 function partially suppressed duplicated fin fold phenotype in ogo mutant embryos. (D,E) Similar experiment in din mutant embryos did not show this effect. (F-I) bmp4 expression was reduced after injection of MOprdm1 in ogo but not din mutants. (J-M) Anterior neural ectoderm was expanded mediolaterally in ogo but not din mutants injected with MOprdm1 (white arrows) compared with untreated siblings (white open arrows). (N,O) Animal-vegetally elongated morphology of morphant embryos compared with control siblings at the end of gastrulation; dimensions measured are indicated, embryonic length (yellow), dorsal mesoderm extension (green), animal-vegetal axis (red), mediolateral axis (blue). (P,Q) Animal-vegetal elongation of morphant embryonic axis persisted throughout early segmentation. (R,S) Forming somites were mediolaterally expanded. (T) Schematic overview of photoactivation of caged lineage tracer dye, dorsal at the embryonic shield and ventral at the blastoderm margin. (U,V) Tracking of dorsally photoactivated lineage tracer (between green arrows) at the end of gastrulation. (W,X) Tracking movements of ventrally photoactivated lineage tracer (between green arrows) at the end of gastrulation. Scale bars: 150 µm in B,D; 200 µm in G,U; 100 µm in R. av, animal-vegetal axis; bm, blastoderm margin; d, dorsal; dm, dorsal mesoderm extension; el, embryonic length; es, embryonic shield; ml, mediolateral axis; s5, fifth somite; v, ventral; vm, ventralmost mesoderm; ye, yolk extension.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bmp4 chrdtt250/tt250 standard conditions Bud unspecified ISH
chrdtt250/tt250 + MO1-prdm1a standard conditions Bud unspecified ISH
szltm305/tm305 standard conditions Bud unspecified ISH
szltm305/tm305 + MO1-prdm1a standard conditions Bud unspecified ISH
dlx3b chrdtt250/tt250 standard conditions Bud ectoderm ISH
chrdtt250/tt250 + MO1-prdm1a standard conditions Bud ectoderm ISH
szltm305/tm305 standard conditions Bud ectoderm ISH
szltm305/tm305 + MO1-prdm1a standard conditions Bud ectoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-prdm1a standard conditions 5-9 somites axis increased length, abnormal
5-9 somites somite increased width, abnormal
chrdtt250/tt250 standard conditions Prim-5 ventral fin fold duplicated, abnormal
Prim-5 ventral fin fold increased size, abnormal
chrdtt250/tt250 + MO1-prdm1a standard conditions Prim-5 ventral fin fold duplicated, abnormal
Prim-5 ventral fin fold increased size, abnormal
szltm305/tm305 standard conditions Prim-5 ventral fin fold duplicated, abnormal
Prim-5 ventral fin fold increased size, abnormal
szltm305/tm305 + MO1-prdm1a standard conditions Prim-5 post-vent region has normal numbers of parts of type ventral fin fold, normal
Prim-5 ventral fin fold increased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development