ZFIN ID: ZDB-FIG-050221-7
Wilm et al., 2005 - Essential roles of a zebrafish prdm1/blimp1 homolog in embryo patterning and organogenesis. Development (Cambridge, England)   132(2):393-404 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Shield to Day 5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Shield to 14-19 somites

Fig. 6 MO-induced interference with Prdm1 activity affects DV patterning. (A,B,G-J,O,P) Lateral view, dorsal to the right. (C-F) Animal view, dorsal to the right. (K,L) Dorsal view, anterior to the top. (M,N) Animal view, dorsal to the bottom. (K,M;L,N) different views of same embryos. (Q-T) Lateral views, anterior to the left; tailbud region is shown. (A,C,E,G,I,K,M,O,Q,S) Untreated controls (ctrl). (B,D,F,H,J,L,N,P) Embryos microinjected with 2 ng of MOprdm1 (MO). (R,T) Embryos microinjected with 1 ng of MOprdm1. (A,B) bmp4 expression. (C,D) chd expression, domain boundaries highlighted (arrows). (E,F) szl expression. (G,H) Reduced ventral expression of bmp4 in morphant smu embryos; lack of ptc1 expression identifies smu mutants (not shown). (I,J) Increased dorsal chd expression in MZoep mutant embryos. (K,L) Mediolateral expansion of forming neural and somitic tissues as revealed by expression of krox20, dlx3b and dlc. (M,N) Formation of the prechordal plate was not altered in morphants. (O,P) AP neural patterning, revealed by six3b, pax2a and krox20 expression. (Q,R) Ventroposterior expression of gata1 in blood precursors. (S,T) Expression of eve1 in ventroposterior cells in the tail tip at midsegmentation. Scale bar: 200 ┬Ám. d, dorsal; r3/rS, rhombomeres 3 and 5; v, ventral. Animal pole highlighted by a black star. The vegetal pole is highlighted by a white star.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bmp4 WT standard conditions 90%-epiboly presumptive cephalic mesoderm ISH
90%-epiboly ventral mesoderm ISH
WT + MO1-prdm1a standard conditions 90%-epiboly presumptive cephalic mesoderm ISH
90%-epiboly ventral mesoderm ISH
smob641/b641 + MO1-prdm1a(AB) standard conditions Bud unspecified ISH
smob641/b641(AB) standard conditions Bud unspecified ISH
chrd WT standard conditions Shield unspecified ISH
WT + MO1-prdm1a standard conditions Shield unspecified ISH
tdgf1tz257/tz257 standard conditions 75%-epiboly unspecified ISH
tdgf1tz257/tz257 + MO1-prdm1a standard conditions 75%-epiboly unspecified ISH
ctslb WT standard conditions 1-4 somites prechordal plate ISH
WT + MO1-prdm1a standard conditions 1-4 somites prechordal plate ISH
dlc WT standard conditions 1-4 somites paraxial mesoderm ISH
WT + MO1-prdm1a standard conditions 1-4 somites paraxial mesoderm ISH
dlx3b WT standard conditions 1-4 somites unspecified ISH
WT + MO1-prdm1a standard conditions 1-4 somites hindbrain neural plate ISH
egr2b WT standard conditions 1-4 somites hindbrain neural plate ISH
5-9 somites unspecified ISH
WT + MO1-prdm1a standard conditions 1-4 somites unspecified ISH
5-9 somites unspecified ISH
eve1 WT standard conditions 14-19 somites tail bud ISH
WT + MO1-prdm1a standard conditions 14-19 somites tail bud ISH
gata1a WT standard conditions 14-19 somites presumptive blood ISH
WT + MO1-prdm1a standard conditions 14-19 somites presumptive blood ISH
pax2a WT standard conditions 5-9 somites midbrain hindbrain boundary neural rod ISH
5-9 somites tail bud ISH
WT + MO1-prdm1a standard conditions 5-9 somites midbrain hindbrain boundary neural rod ISH
prdm1a WT standard conditions 20-25 somites slow muscle cell ISH
Prim-5 slow muscle cell ISH
Day 5 lateral line system ISH
six3b WT standard conditions 5-9 somites forebrain neural rod ISH
WT + MO1-prdm1a standard conditions 5-9 somites forebrain neural rod ISH
szl WT standard conditions Shield unspecified ISH
WT + MO1-prdm1a standard conditions Shield unspecified ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-prdm1a standard conditions Shield - 14-19 somites dorsal/ventral pattern formation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development