FIGURE SUMMARY
Title

Behavioral Effects of Developmental Exposure to JWH-018 in Wild-Type and Disrupted in Schizophrenia 1 (disc1) Mutant Zebrafish

Authors
García-González, J., de Quadros, B., Havelange, W., Brock, A.J., Brennan, C.H.
Source
Full text @ Biomolecules
Figure 1

Experimental timeline for developmental exposure to 1-pentyl-3-(1-naphthoyl)indole (JWH-018), delta-9-tetrahydrocannabinol (THC), and nicotine. The behavioral tests performed are represented in light blue.

Figure 2

(A) Forced light/dark test in 5 days post fertilization (dpf) zebrafish larvae. Sample size: n = 64 for each dose group. Each dot represents mean distance travelled per minute. Error bars represent ± SEM. (B,C) One-second resolution plots of the transitions from dark to light during the forced light/dark test. (D) Response and habituation to acoustic startle in 6 dpf zebrafish larvae. Sample sizes: control: n = 87, JWH-018-treated: n = 81. Each dot represents mean distance travelled per second. Error bars represent ± SEM.

Figure 3

(A) Forced light/dark test in wild-type zebrafish exposed to 2 μM THC. Sample size: n = 48 for each dose group. Each dot represents the mean of the total distance travelled per minute. Error bars represent ± SEM. (B,C) One-second resolution plots of the transitions from dark to light during the forced light/dark test. (D) Distances travelled by control and THC-treated larvae before and after exposure to 10 sound/vibration stimuli. Figure shows mean distances travelled in one-second time bins. Each dot represents mean distance travelled per second.Error bars represent ± SEM. Sample sizes: n = 48 per dose group.

Figure 4

(A) Forced light/dark test in wild-type zebrafish exposed to 0.15 μM nicotine. Sample size: n = 48 for each dose group. Each dot represents the mean of the total distance travelled per minute. Error bars represent ± SEM. (B,C) One-second resolution plots of the transitions from dark to light during the forced light/dark test. (D) Distances travelled by control and nicotine-treated larvae before and after exposure to 10 sound/vibration stimuli. Figure shows mean distances travelled in one-second time bins. Each dot represents mean distance travelled per second. Error bars represent ± SEM. Control: n = 23, treated with 0.15 μM nicotine: n = 23.

Figure 5

(A) Forced light/dark test in 5 dpf wild-type and disc1 loss-function mutant larvae. Sample sizes for each group: control disc1 +/+: n = 30, JWH-018 disc1 +/+: n = 34, control disc1 +/–: n = 33, JWH-018 disc1 +/–: n = 27, control disc1 –/–: n = 107, JWH-018 disc1 –/–: n = 92. Each dot represents mean distance travelled per minute. Error bars represent ± SEM. (B,C) One-second resolution plots of the transitions from dark to light during the forced light/dark test. Each dot represents mean distance travelled per second. (D) Response and habituation to startle stimuli test in 6 dpf control and JWH-018-treated wild-type and disc1 mutant larvae. Sample sizes: control disc1 +/+: n = 15, JWH-018 disc1 +/+: n = 13, control disc1 +/–: n = 47, JWH-018 disc1 +/–: n = 47, control disc1 –/–: n = 22, JWH-018 disc1 –/–: n = 22.

Figure 6

(A) Forced light/dark test in 5 dpf wild-type and disc1 loss-function mutant larvae. Sample sizes for each group: control disc1 +/+: n = 39, THC disc1 +/+: n = 29, control disc1 +/–: n = 41, THC disc1 +/–: n = 45, control disc1 –/–: n = 40, THC disc1 –/–: n = 29. Each dot represents mean distance travelled per minute. Error bars represent ± SEM. (B,C) One-second resolution plots of the transitions from dark to light during the forced light/dark test. Each dot represents mean distance travelled per second. (D) Response and habituation to startle stimuli test in 6 dpf control and THC-treated wild-type and disc1 mutant larvae. Sample sizes: control disc1 +/+: n = 36, THC disc1 +/+: n = 35, control disc1 +/–: n = 36, THC disc1 +/–: n = 35, control disc1 –/–: n = 36, THC disc1 –/–: n = 36.

Figure 7

Novel tank diving response in adult wild-type and mutant disc1 zebrafish after developmental exposure to 3 μM JWH-018. (A) Time spent on the bottom of the tank, (B) Cumulative distance travelled (C) Number of transitions between the top and bottom of the tank. Sample sizes for each group: control disc1 +/+: n = 23, JWH-018 disc1 +/+: n = 17, control disc1 +/–: n = 35, JWH-018 disc1 +/–: n = 34, control disc1 –/–: n = 15, JWH-018 disc1 –/–: n = 19. Error bars represent ± SEM.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomolecules
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