(A) Forced light/dark test in wild-type zebrafish exposed to 0.15 μM nicotine. Sample size: n = 48 for each dose group. Each dot represents the mean of the total distance travelled per minute. Error bars represent ± SEM. (B,C) One-second resolution plots of the transitions from dark to light during the forced light/dark test. (D) Distances travelled by control and nicotine-treated larvae before and after exposure to 10 sound/vibration stimuli. Figure shows mean distances travelled in one-second time bins. Each dot represents mean distance travelled per second. Error bars represent ± SEM. Control: n = 23, treated with 0.15 μM nicotine: n = 23.
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