Fig. 4

Temporal mRNA expression of glypicans genes during early embryonic development.

RT-PCR was performed for all 10 genes using cDNA from specified stages of development. β-actin was used as a control. Ep, epiboly; som, somites; hpf, hours post fertilization.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: 8-cell to Prim-5
Fig. 5

Spatial expression of zebrafish glypicans during cleavage to early segmentation stages.

In situ hybridization was performed to detect glypican mRNA during early developmental stages. Lateral views with anterior to the top (A, B, C) and dorsal to the right (B, C). Developmental stages and gene names are indicated. tb, tailbud; hb, hindbrain; en, endoderm; me, mesencephalon; n.d: not detected.

Fig. 6

Spatial expression pattern of zebrafish glypicans during segmentation and pharyngula stages.

In situ hybridization was carried out to detect glypican mRNA at 20 somite stage: 19 hpf and pharyngula stages: 24-26 hpf. Lateral view of whole mount embryos, anterior to the left. Expression pattern of gpc1a (A, A′), gpc1b (B, B′); subfigure in B is an optical section indicating expression in yolk cells (arrowheads) and branchial arch primordium, gpc2 (C, C′), gpc3 (D, D′), gpc4 (E, E′), gpc5a (F, F′), gpc5b (G, G′), gpc5c (H, H′), gpc6a (I, I′), gpc6b (J, J′). di, diencephalon; te, telencephalon; hb, hindbrain; tg, tegmentum; nc, neural crest; pm, paraxial mesoderm; som, somites; ba, branchial arch; y, yolk; mb, midbrain; ff, fin fold; ov, otic vesicle; fp, floor plate; psom, posterior somites; ep, epiphysis; tri, trigeminal placode; cl, cloaca; pllp, posterior lateral line primordium; cg, cranial ganglion.
Fig. 7

Specific expression domains for gpc1a, gpc1b and gpc5b.

Transverse cross sections revealed the expression details of gpc1a, gpc1b and gpc5b. Dotted line indicates the plane of section. pllp: posterior lateral line primordium; pba: posterior branchial arch; my: myotome; fp: floor plate; nc: notochord.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.