In situ hybridization was carried out to detect glypican mRNA at 20 somite stage: 19 hpf and pharyngula stages: 24-26 hpf. Lateral view of whole mount embryos, anterior to the left. Expression pattern of gpc1a (A, A′), gpc1b (B, B′); subfigure in B is an optical section indicating expression in yolk cells (arrowheads) and branchial arch primordium, gpc2 (C, C′), gpc3 (D, D′), gpc4 (E, E′), gpc5a (F, F′), gpc5b (G, G′), gpc5c (H, H′), gpc6a (I, I′), gpc6b (J, J′). di, diencephalon; te, telencephalon; hb, hindbrain; tg, tegmentum; nc, neural crest; pm, paraxial mesoderm; som, somites; ba, branchial arch; y, yolk; mb, midbrain; ff, fin fold; ov, otic vesicle; fp, floor plate; psom, posterior somites; ep, epiphysis; tri, trigeminal placode; cl, cloaca; pllp, posterior lateral line primordium; cg, cranial ganglion.