- Title
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Enhancer detection and developmental expression of zebrafish sprouty1, a member of the fgf8 synexpression group
- Authors
- Komisarczuk, A.Z., Topp, S., Stigloher, C., Kapsimali, M., Bally-Cuif, L., and Becker, T.S.
- Source
- Full text @ Dev. Dyn.
In situ hybridization with spry1 antisense probe on wild-type embryos. A-L: At the 2-somite stage (A), 18-somite stage (B), 22 hours postfertilization (hpf; C-E,F′), 48 hpf (F-H), and 120 hpf (I-L). A-C,F,I,J: Lateral view, anterior to the left (I, anterior to the bottom); C′,H,L: ventral view, anterior to the top; D,E,F′,G,K: dorsal view, anterior to the top. spry1 expression domains are similar to the fgf8a expression pattern. Note in F′ (spry1-blue, ephA4-red marks r3 and r5) that rhombomere expression is not in register with rhombomere boundaries, but rather corresponds to rhombomere centers. BA, branchial arches; CF, caudal fin fold; CLLn, cranial lateral line neuromasts; D, dorsal diencephalon; G, gills, H, hyoid; HB, hindbrain; MHB, midbrain-hindbrain boundary; N, notochord; NC, neural crest; ON, optic nerve; OP, olfactory placodes; OS, optic stalk; OV, otic vesicle; PF, pectoral fin; PLLg, posterior lateral line ganglion; PLLn, posterior lateral line neuromasts; PLLp, posterior lateral line primordium; PM, paraxial mesoderm; PNP, posterior neural plate; PR, pronephros; S, somites; SC, spinal cord; R, retina; Rh, rhombomeres; RhC, rhombomere centers; T, telencephalon; Ter, terminal neuromasts; TT, tail tip. EXPRESSION / LABELING:
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YFP expression pattern in CLGY786 line mimics endogenous spry1. A-L: At the 2-somite stage (A), 16-somite stage (B), 22 hours postfertilization (hpf; C-E), 48 hpf (F-H), and 120 hpf (I-L). A-C,F,I,J: Lateral view, anterior to the left (I, anterior to the bottom); C′,H,L: ventral view, anterior to the top; D,E,G,K: dorsal view, anterior to the top. P, pituitary; CS, craniofacial skeleton; M, muscle. For other abbreviations, see Figure 2. EXPRESSION / LABELING:
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Immunostaining of the CLGY786/spry1 line shows domains where YFP protein is expressed. A-L: At the 2-somite stage (A), 18-somite stage (B), 22 hours postfertilization (hpf; C-E), 48 hpf (F-H), and 120 hpf (I-L). A-C,F,I,J: Lateral view, anterior to the left (I, anterior to the bottom); C′,H,L: ventral view, anterior to the top; D,E,G,K: dorsal view, anterior to the top. For abbreviations, see Figures 2 and 3. EXPRESSION / LABELING:
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Immunostaining of the CLGY786/spry1 line, cross-sections of anterior regions show domains where YFP protein is expressed. A-E: At 3 hours postfertilization (hpf). F-J: At 6 hpf. ALLg, anterior lateral line ganglion; cec, cerebellar commissure; Ch, chorda dorsalis; E, epiphysis; Ep, epithelium; FG, facial ganglion; mlf, medial longitudinal fascicle; OC, otic capsule; OG, otic ganglion; OSC, otic support cells; Ot, otolith; Po, preoptic region; TG, trigeminal ganglion; VA, visceral arches. For other abbreviations, see Figure 2 and 3. EXPRESSION / LABELING:
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Sprouty1 and the Fgf signaling pathway. A,B: Twenty-four hour postfertilization stage. Loss of fgf8a in ace inhibits spry1 expression. In situ hybridization with spry1 antisense probe in wild-type (wt) (A) and ace fgf8a-/- mutant (B) embryos. C-J: Wild-type embryos treatment with the inhibitor of FGF signaling SU5402 commenced at the18-somite stage. Incubation for 1 hr (C,D), 6 hr (E,F), 12 hr (G,H), and 16 hr (I,J). The asterisk indicates midbrain-hindbrain boundary (MHB) position and arrowhead the tail tip. Expression of spry1 is inhibited after 1 hr of incubation in most of the domains, and after 16 hr of incubation there is no detectable expression of spry1. EXPRESSION / LABELING:
PHENOTYPE:
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