ZFIN Image: Chen et al., 2025, FIGURE 8

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Figure Caption

FIGURE 8

Wnt signaling disruption in ddx3xa^−/− zebrafish. (A) Expression of key Wnt signaling components in ddx3xa^−/− larvae at 72 hpf by qRT-PCR (n = 3; *p < 0.05, **p < 0.01, ***p < 0.001 vs. WT). (B) Whole-mount in situ hybridization (WISH) with tbx5 probe at 48 hpf. Numbers indicate identically stained embryos/total processed. Scale bar: 250 μm (applies to B–C). (a) WT dorsal view; (b) ddx3xa^−/− dorsal view; (C) WT lateral view; (D)ddx3xa^−/− lateral view. (C) WISH with bmp4 probe at 48 hpf. (a) WT ventral view; (b) ddx3xa^−/− ventral view. (D)bmp4 expression levels in ddx3xa^−/− embryos at 24, 48, and 96 hpf by qRT-PCR (n = 3; ***p < 0.001 vs. WT; ns: nonsignificant). (E)tbx5 expression levels in ddx3xa^−/− embryos at 24, 48, and 96 hpf by qRT-PCR (n = 3; *p < 0.05, ***p < 0.001 vs. WT).

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Acknowledgments

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