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Fig. 5 Differentiation and mature adipocyte markers are downregulated in fstl1a KO larvae under high fat diet compared to wild types. a) Table showing the expression analysis for different genes associated with preadipocytes, adipogenesis (differentiation), mature adipocytes, and activation of the Shh signalling pathway. qPCR results were analysed using a two-way ANOVA to evaluate the effect of diet and genotype on the expression of the different genes. The table indicates the p-values of the simple main effect analysis and the interaction between diet and genotype for each gene. b) Dot plots with the individual NRQ values and the mean ± CI95% of mRNA for different preadipocyte (dlk1, zfp423), differentiation (pparg1, pparg2, cebpa) and mature adipocyte (fabp4a, adipoqb, pln2) marker genes, as well as genes indicative of Shh pathway activation (gli1, ptch1, ptch2) in the abdominal region of wild-type (WT; blue) or fstl1a KO (KO; magenta) larvae under standard (?; empty circle) or high fat diet (?; full circle). Five larvae from one diet experiment were analysed in each condition. Significative pairwise differences are indicated with their p-values (Tuckey post-hoc test).