PUBLICATION
Follistatin like-1 (Fstl1) regulates adipose tissue development in zebrafish
- Authors
- Guggeri, L., Sosa-Redaelli, I., Cárdenas-Rodríguez, M., Alonso, M., González, G., Naya, H., Prieto-Echagüe, V., Lepanto, P., Badano, J.L.
- ID
- ZDB-PUB-241208-18
- Date
- 2024
- Source
- Adipocyte 13: 24358622435862 (Journal)
- Registered Authors
- Badano, Jose, Cárdenas-Rodríguez, Magdalena, Lepanto, Paola
- Keywords
- Adipose tissue, Bardet?Biedl syndrome, cilia, follistatin-like 1, zebrafish
- MeSH Terms
-
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish*
- Cilia/metabolism
- Follistatin-Related Proteins*/genetics
- Follistatin-Related Proteins*/metabolism
- Adipogenesis/genetics
- Cell Differentiation
- Adipose Tissue*/metabolism
- Adipocytes/cytology
- Adipocytes/metabolism
- Animals
- PubMed
- 39644214 Full text @ Adipocyte
Citation
Guggeri, L., Sosa-Redaelli, I., Cárdenas-Rodríguez, M., Alonso, M., González, G., Naya, H., Prieto-Echagüe, V., Lepanto, P., Badano, J.L. (2024) Follistatin like-1 (Fstl1) regulates adipose tissue development in zebrafish. Adipocyte. 13:24358622435862.
Abstract
Obesity is a highly prevalent disorder with complex aetiology. Therefore, studying its associated cellular and molecular pathways may be aided by analysing genetic tractable diseases. In this context, the study of ciliopathies such as Bardet-Biedl syndrome has highlighted the relevance of primary cilia in obesity, both in the central nervous system and peripheral tissues. Based on our previous in vitro results supporting the role of a novel Bbs4-cilia-Fstl1 axis in adipocyte differentiation, we evaluated the in vivo relevance of the zebrafish orthologous genes fstl1a and fstl1b in primary cilia and adipose tissue development. Using a combination of knockdowns and a new fstl1a mutant line, we show that fstl1a promotes primary cilia formation in early embryos and participates in adipose tissue formation in larvae. We also show that fstl1b partially compensates for the loss of fstl1a. Moreover, in high fat diet, fstl1a depletion affects the expression of differentiation and mature adipocyte markers. These results agree with our previous in vitro data and provide further support for the role of FSTL1 as a regulator of adipose tissue formation. Dissecting the exact biological role of proteins such as FSTL1 will likely contribute to understand obesity onset and presentation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping