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Figure 3
Treatment of HSF1 MO (morpholino) significantly reduced the mRNA expression of Klf4, Oct4, and Sox2 6 h after ONI. (a) HSF1 MO or standard MO (Std. MO) was injected intraocularly 20 h before ONI. (b) HSF1 MO-treated group suppressed HSF1 mRNA expression compared to the Std. MO-treated group. Under these conditions, the mRNA expression of Klf4 (c), Oct4 (d), and Sox2 (e) was inhibited compared to the control (Std. MO) groups. Five experiments were repeated under each experimental condition. Data are expressed as the mean ± SEM of independent experiments and analyzed by one-way ANOVA, followed by Scheffe?s multiple comparisons. Statistical significance was set at * p < 0.05.