ZFIN Image: Xia et al., 2022, Fig. 3

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Figure Caption

Fig. 3 a Normalized expression of marker genes on UMAPs (3 samples combined). b Heatmap of 3 marker genes: tcf21, aldh1a2, and hapln1a. Normalized expression values are shown; cells were sorted by cluster membership. Clusters 2 and 5 are highlighted in red frames. c Images of a 3 dpa heart section showing the ventricular wall with tcf21:nucEGFP in magenta and HCR staining of podxl in green. Nuclei were stained with DAPI (blue). A single-channel image of podxl is shown in grayscale on the right. Arrows indicate representative EGFP⁺podxl⁺ cells. Scale bar, 50 μm. d Images of a 3 dpa heart section showing the ventricular wall with tcf21:nucEGFP in magenta and HCR staining of hapln1a in green. Nuclei were stained with DAPI (blue). A single-channel image of hapln1a is shown in grayscale on the right. Arrows indicate representative EGFP⁺hapln1a⁺ cells. Scale bar, 50 μm. e Normalized expression levels of marker genes for the 3 dpa-specific cluster on UMAPs (3 samples combined).

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Acknowledgments

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