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Fig. 8 The otofB mutation results in abnormal glutamate decarboxylase and GABA receptor subunit transcripts levels. (A–C) Quantification of mRNA by qRT-PCR for GABA receptor subunits and GAD transcripts in wild-type and otofB larvae at 72, 96, and 120 hpf. N = 4 for both WT and mutant, with each N as a composite of 20–30 larvae. (D) Quantification of mRNA by qRT-PCR for GABA receptor subunit and GAD transcripts in wild-type and otofB adult zebrafish brain (t test, p < 0.001). N = 4 for both WT and mutant, with each N as a composite of 20–30 larvae. The data presented are representative of three experimental replicates for each time point. Error bars are SEM.

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