ZFIN Image: Cavone et al., 2021, Fig. 2

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Figure Caption

Fig. 2 tnfa+ macrophages have a unique signature after injury (A) Unsupervised clustering of mpeg1:GFP+ cells at 1 dpl shows multiple clusters of cell identities. (B) Macrophages and microglia express mpeg1 and mfap4, but macrophages express lower levels of apoeb and p2ry12 than microglia. (C) tnfa is mainly expressed in macrophages. tnfa and slc7a7 are upregulated in macrophages after injury. (D) Unsupervised clustering of tnfa+ macrophages identifies two clusters in unlesioned and lesioned samples. Expression of fth1a identifies clusters of “activated macrophages.” For clarity, only cells with expression of > 1 are indicated in feature plot of fth1a. (E) Heatmaps of differentially expressed genes between tnfa+ macrophages. Top 10 genes shown for the indicated categories (see also Figure S1; Tables S1–S5).

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Acknowledgments

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Reprinted from Developmental Cell, 56(11), Cavone, L., McCann, T., Drake, L.K., Aguzzi, E.A., Oprişoreanu, A.M., Pedersen, E., Sandi, S., Selvarajah, J., Tsarouchas, T.M., Wehner, D., Keatinge, M., Mysiak, K.S., Henderson, B.E.P., Dobie, R., Henderson, N.C., Becker, T., Becker, C.G., A unique macrophage subpopulation signals directly to progenitor cells to promote regenerative neurogenesis in the zebrafish spinal cord, 1617-1630.e6, Copyright (2021) with permission from Elsevier. Full text @ Dev. Cell