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Cabrera-Quio et al., 2021 - Zebrafish Ski7 tunes RNA levels during the oocyte-to-embryo transition

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Fig 2 Zebrafish Ski7 interacts with the cytoplasmic exosome.

(A) mRNA expression levels of ski7 (purple), subunits of the cytoplasmic RNA exosome (orange) and the SKI complex (green). PolyA⁺ RNA-seq during oogenesis (oocyte stages I-IV), mature eggs (unactivated, activated, fertilized) and embryogenesis (2–4 cell, 256 cell, 1000 cell, oblong, dome, shield, bud, 1 dpf, 2 dpf, 5 dpf [34]); TPM, transcripts per million. (B) Ski7 associates with the cytoplasmic exosome in early embryos. Volcano plot of Ski7-P4-like peptide-interacting proteins from wild-type zebrafish embryo lysates (128-512-cell stage) identified by mass spectrometry. Subunits of the cytoplasmic exosome (orange) are enriched, while nuclear exosome auxiliary factors (blue) and the SKI complex (green) are not enriched.

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