IMAGE

Fig. 6

ID
ZDB-IMAGE-190626-15
Genes
Source
Figures for Gerber et al., 2019
Image
Figure Caption

Fig. 6

Knockout of sox1a and sox1b causes increase of V2b cells. (A-A″) Knockout of sox1aand sox1b increases gata2a+ cells in the V2 domain by 22%. (B-B″) tal1+ cells are increased by 83% in sox1a−/−; sox1b−/− embryos. (C-C″) Lack of sox1a/b results in an increase of tal2+ cells by 75%. (D-D″) The sox1a/b mutant has 35% more gata3+ cells. (E-E″) vsx2+ cells are not affected by the mutations. (F-F″) At 30 hpf, the gata3+ cells in the V2 domain are increased by 2.3-fold. (G-I) Double FISH of nkx1.2lb (red) and slc6a5 (green) at 30 hpf and quantification of nkx1.2lb+; slc6a5+ cells in I. Glycinergic nkx1.2lb+ cells were decreased by 22% in the mutant. Asterisks indicate double-labelled cells. Counts of gata2a+ cells (A-A″) were derived from the fourth somite to the tail on both spinal cord sides from five embryos. Counts of gata3-, tal2-, tal1-, vsx2- and nkx1.2lb; slc6a5-expressing cells were derived from the spinal cord above the yolk extension over five somites from three to eight embryos (B-F″). V2 cells, white arrowheads (A-F′). Wild-type (white) and mutant (black) values are presented as mean±s.e.m. in A″-F″,I from at least two independent experiments. Statistical significance was assessed using the unpaired two-tailed Student's t-test. *P≤0.05, **P≤0.01, ***P≤0.001. Lateral views at 24 hpf (A-E′) and 30 hpf (F-H″). Dorsal is upwards; anterior is leftwards. Scale bars: 25 µm.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development