PUBLICATION

The HMG box transcription factors Sox1a and b specify a new class of glycinergic interneurons in the spinal cord of zebrafish embryos

Authors
Gerber, V., Yang, L., Takamiya, M., Ribes, V., Gourain, V., Peravali, R., Stegmaier, J., Mikut, R., Reischl, M., Ferg, M., Rastegar, S., Strähle, U.
ID
ZDB-PUB-190215-5
Date
2019
Source
Development (Cambridge, England)   146(4): (Journal)
Registered Authors
Ferg, Marco, Gerber, Vanessa, Gourain, Victor, Mikut, Ralf, Peravali, Ravindra, Rastegar, Sepand, Strähle, Uwe, Takamiya, Masanari, Yang, Lixin
Keywords
Axonal projections, Development, Glycinergic neurotransmission, Mouse V2c interneuron, Notch, Spinal cord, Transcriptional network, Zebrafish, gata2a, gata3, sox1a, sox1b
MeSH Terms
  • Motor Neurons/metabolism*
  • Green Fluorescent Proteins/metabolism
  • Species Specificity
  • Mice, Transgenic
  • Intracellular Signaling Peptides and Proteins/metabolism
  • Signal Transduction
  • Homeodomain Proteins/metabolism
  • Mutation
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Interneurons/metabolism*
  • Gene Expression Regulation, Developmental*
  • Behavior, Animal
  • Genotype
  • GATA2 Transcription Factor/metabolism
  • Receptors, Notch/metabolism
  • Animals
  • Zebrafish Proteins/metabolism
  • Mice
  • SOXB1 Transcription Factors/metabolism*
  • Spinal Cord/embryology
  • Spinal Cord/metabolism*
  • Glycine/chemistry
(all 23)
PubMed
30760481 Full text @ Development
Abstract
Specification of neurons in the spinal cord relies on extrinsic and intrinsic signals, which in turn are interpreted by expression of transcription factors. V2 interneurons develop from the ventral aspects of the spinal cord. We report here a novel neuronal V2 subtype, named V2s, in zebrafish embryos. Formation of these neurons depends on the transcription factors sox1a and sox1b. They develop from common gata2a/gata3 dependent precursors co-expressing markers of V2b and V2s interneurons. Chemical blockage of Notch signaling causes a decrease of V2s and an increase of V2b cells. Our results are consistent with the existence of at least two types of precursors arranged in a hierarchical manner in the V2 domain. V2s neurons grow long ipsilateral descending axonal projections with a short branch at the ventral midline. They acquire a glycinergic neurotransmitter type during the second day of development. Unilateral ablation of V2s interneurons causes a delay in touch-provoked escape behavior suggesting that V2s interneurons are involved in fast motor responses.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
fr7
    Point Mutation
    ka7TgTransgenic Insertion
      ka701
        Small Deletion
        ka702
          Indel
          ka705TgTransgenic Insertion
          nns5TgTransgenic Insertion
            vu12TgTransgenic Insertion
              1 - 7 of 7
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              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              1 - 6 of 6
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              Fish
              Antibodies
              Name Type Antigen Genes Isotypes Host Organism
              Ab1-gadpolyclonal
                Rabbit
                1 - 1 of 1
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                Orthology
                No data available
                Engineered Foreign Genes
                Marker Marker Type Name
                EGFPEFGEGFP
                GFPEFGGFP
                1 - 2 of 2
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                Mapping
                No data available