ZFIN Image: Sun et al., 2018, Fig. 3

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Fig. 3

Involvement of Cftr in Wnt-dependent hematopoiesis in zebrafish.

WISH showing impaired wnt3a-induced expression of scl and gata1a in wnt3a mRNA and cftr morpholino co-injected zebrafish (a) and decreased expression of cdx4 and hoxa9a in cftr mutants (b). c Quantitative RT-PCR showing significantly reduced c-myc and lef1 mRNA levels in cftr mutants. d CFTR knockdown by siRNA attenuated the Wnt reporter TopFlash activity in HEK293 cells (n = 3). e Western blot analysis showing reduced Dvl2, 3 and nuclear β-catenin expression in cftr mutants. f Quantitative RT-PCR showing no significant changes in dvl2, 3 mRNA levels in cftr mutants. g Western blot analysis showing increased Dvl1-3 protein levels in co-expressing Cftr with Dvl1-3 HEK293 cells as compared to Dvl1-3 alone controls. h Effects of dvl2 mRNA in rescuing hematopoietic defects in cftr mutants. Embryos shown in a, b, and h are dorsal views with anterior to the top at 8-somite stage (13 hpf)

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Acknowledgments

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