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van der Vaart et al., 2013 - Functional analysis of a zebrafish myd88 mutant identifies key transcriptional components of the innate immune system

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Fig. 4 qPCR analysis of the response of myd88^-/- and wild-type embryos to E. tarda or S. typhimurium infection.
Expression of il1b, tnfa, il8, cxcl-c1c, mmp9 and ifnphi1 was analyzed by qPCR in wild-type (Wt) and mutant (Mu) embryos infected with E. tarda (A) or S. typhimurium (B). RNA samples from infected embryos (inf) and uninfected controls were taken 8 hpi. Each data point represents an individual embryo and lines indicate the mean relative expression level, with uninfected wild-type set at 1. Significant differences were calculated by one-way ANOVA with Tukey’s multiple comparison method as a post-hoc test; *P<0.05; **P<0.01; ***P<0.001; ns, not significant.

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