FIGURE

Fig. 6.

ID

ZDB-FIG-260522-51

Publication

Dockery et al., 2026 - Genes for 5'mRNA cap binding proteins, eif4ea and eif4eb, have alternative roles during heart regeneration

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Fig. 6.

An increased ratio of eif4e1c to canonical cap binding alleles improves heart regeneration. (A) Hearts, 7 days after amputation of the apex and after 3 days injection of EdU. CM are stained with α-Mef2c. (B) The single Δeif4ea mutants (green) and the Δeif4eb mutants (purple) have increased levels of CM proliferation (Mean: wildtype = 9.6%, Δeif4ea = 12.9%, Δeif4eb = 12.8%, Δeif4ea/Δeif4eb = 8.1%, ANOVA p < 0.0001, N = 48, 37, 40, 38). However, after a certain threshold, eif4e1c fully compensates since Δeif4ea/Δeif4ea double mutants (pink) regenerate like their wildtype (black) siblings. (C) Graph of fin regrowth 2 days post amputation in the respective mutants (Mean: wildtype = 244um, Δeif4ea/Δeif4eb = 270um, Δeif4ea = 194um, Δeif4eb = 183um, ANOVA p-value <0.0001, N = 50, 73, 41, 42). (D) Images of representative fins from the graph. Error bars representmean ± s.e.m. Scale bar = 100um. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Expression Data

Antibody:	Ab5-mef2
Fish:	eif4ea^os81/os81 eif4eb^os83/os83 eif4eb^os84/os84 eif4ea^os81/os81; eif4eb^os83/os83 eif4ea^os81/os81; eif4eb^os84/os84
Condition:	amputation: cardiac ventricle
Anatomical Terms:	heart regenerating tissue regenerating fin
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	eif4ea^os81/os81 eif4eb^os83/os83 eif4eb^os84/os84 eif4ea^os81/os81; eif4eb^os83/os83 eif4ea^os81/os81; eif4eb^os84/os84
Condition:	amputation: cardiac ventricle
Observed In:	cardiac muscle cell cell division heart regenerating tissue regenerating fin
Stage:	Adult

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 534, Dockery, R., Zevallos, M., McNulty, C., Zecchino, T., Ratermann, R., White, R., Kolonay, D., Baskin, K., Goldman, J.A., Genes for 5'mRNA cap binding proteins, eif4ea and eif4eb, have alternative roles during heart regeneration, 50-60, Copyright (2026) with permission from Elsevier. Full text @ Dev. Biol.