FIGURE

Fig. 5.

ID

ZDB-FIG-260522-50

Publication

Dockery et al., 2026 - Genes for 5'mRNA cap binding proteins, eif4ea and eif4eb, have alternative roles during heart regeneration

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Fig. 5.

Loss of additional alleles from the genes encoding canonical cap binding factors demonstrates a dose dependence in their compensation abilities. (A) Weights of Δeif4eb/Δeif4e1c double mutants compared to of Δeif4eb/Δeif4e1c double/eif4ea hets (Mean: wildtype = 0.287g, Δeif4ea = 0.315g, Δeif4eb/ Δeif4e1c = 0.110g, Δeif4ea-HET/Δeif4eb/Δeif4e1c = 0.039g; ANOVA wt - Δeif4ea p-value = 0.712, ANOVA wt- Δeif4eb/Δeif4e1c p value < 0.0001; N = 47, 37, 34, 14). (B) Lengths of respective mutants (Mean: wildtype = 27.5 mm, Δeif4eb/Δeif4e1c = 20.5 mm, Δeif4ea-HET/Δeif4eb/Δeif4e1c = 15.1 mm, ANOVA p value < 0.001, N = 48, 34, 14). (C)Cardiomyocyte counts from Mef2c positive cells (Mean: wildtype = 2882, Δeif4eb/Δeif4e1c = 1962, Δeif4ea-HET/Δeif4eb/ Δeif4e1c = 1046, ANOVA p value < 0.0001, N = 9, 10, 9). (D) Images of representative hearts. (E) Time course of triple mutant survival. The Δeif4eb/Δeif41c double mutants die by 30 days post fertilization (dpf). Removing each additional eif4ea allele increases death further with triple mutants being lethal by 30dpf showing that the Δeif4ea/Δeif4eb survival depends on eif4e1c. Error bars represent mean ± s.e.m. Scale bar = 100um.

Expression Data

Antibody:	Ab5-mef2
Fish:	eif4e1c^pd370/pd370; eif4ea^os81/+; eif4eb^os83/os83 eif4e1c^pd370/pd370; eif4ea^os81/+; eif4eb^os84/os84
Anatomical Term:	heart
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	eif4e1c^pd370/pd370; eif4ea^os81/+; eif4eb^os83/os83 eif4e1c^pd370/pd370; eif4ea^os81/+; eif4eb^os84/os84
Observed In:	cardiac muscle cell heart whole organism
Stage:	Adult

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 534, Dockery, R., Zevallos, M., McNulty, C., Zecchino, T., Ratermann, R., White, R., Kolonay, D., Baskin, K., Goldman, J.A., Genes for 5'mRNA cap binding proteins, eif4ea and eif4eb, have alternative roles during heart regeneration, 50-60, Copyright (2026) with permission from Elsevier. Full text @ Dev. Biol.