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Spatiotemporal pattern of mymx mRNA expression. (A) Fluorescence in situ hybridisation for mymx mRNA expression (magenta, top panel) in wild-type embryos fixed at 24 hpf co-stained with DAPI (green, bottom panel) (scale bars: 50 µm). (B) Transverse sections of myotomes showing mymx expression at different somite stages (scale bars: 20 µm). (C) Quantification of fluorescence intensities of mymx expression at different somite stages. Error bars represent the standard deviation (n=3 embryos). (D-F) Spatial distribution of mymx expression in myotomes at different somite stages along the anteroposterior (D), dorsoventral (E), and mediolateral (F) axes. The solid lines represent the mean and shaded areas represent the standard deviation (n=3 embryos).
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