Skeletal structure of wild-type and fusion mutant adult zebrafish. (A) Diagram of the adult zebrafish skeleton in lateral view. Coloured elements correspond to the position of skeletal elements depicted in C (skull, purple), D (pectoral girdle, blue), E (precaudal vertebrae, green), F (1st caudal vertebrae cross-section view, yellow) and G (muscle mass comparison, orange). (B) Whole-body comparisons of 3-month-old wild-type, mymk−/− and mymx−/− fish cleared and stained with Alizarin Red. (C) Comparison images of the horizontal view of the skull. Solid white lines trace the shape of the cranium, beginning behind the premaxilla. Dotted line represents the traced shape of the cranium in the mymk−/− fish compared to wild-type. White arrowhead points to abnormal mandibular structure in mymk−/− fish. (D) Comparison of the pectoral girdle size and structure. The white arrowhead indicates left-right asymmetry in the pectoral girdle of mymx−/− mutants. (E) Horizontal view of precaudal vertebrae and supraneural bones compared across genotypes. White rings indicate overgrowth of neural spines of precaudal vertebrae and increased supraneural/ectopic bone growth in mymk−/− and mymx−/− fish. (F) Comparison images of the 1st caudal vertebra. White arrows indicate fusion. (G) Muscle mass size of 3-month-old zebrafish. White arrows indicate the overall bulk of muscle behind the skull. n=3 fish for each genotype.
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