Fig. 3 - Supplemental 2

Quantification of neurite outgrowth across zebrafish mutant lines. (A) Boxplots showing neurite length distributions at 10 and 24 hap for each founder of the microexon deletion lines. The main founder (shown in Figure 3C) is highlighted in bold. Red boxplots correspond to neurons from a Del x Del cross for each microexon line. Dark boxplots correspond to matched control neurons from WT HuC:GFP fish processed in parallel in each experiment (see Materials and methods). (B) Boxplots showing neurite length distributions at 10 and 24 hap for the main founder of the microexon deletion lines. The second founder was not tested since no significant differences were found in any of the initial replicates with the exception of reln, for which no second founder could be obtained. (C) Boxplots showing neurite length distributions at 10 and 24 hap for siblings of WT (blue), heterozygous (yellow), or homozygous (red) genotype. These samples were generated through Het x Het crosses, fin clip genotyping of each larva and processing of pools of larvae of the same genotype. p-Value corresponds to ANOVA tests for each of the groups. * 0.01

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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