Figure 7

Co-expression of the miR-183 family leads to abnormal migration of myocardial precursor cells. (A–C) Fluorescence images of the hearts of Tg (cmlc2: eGFP) transgenic zebrafish embryos at 48 hpf (ventral view). Control embryos exhibit a normally fused heart tube (A) (green). Both miR-183/96/182 mimics OE (B) and miR-183/96/182 capped RNA OE (C) embryos exhibit cardia bifida (white arrow heads indicate separate cardiac primordia). (D,E) Quantification of cardia bifida in miR-183 cluster mimics OE (D) and miR-183/96/182 capped RNA OE (E) embryos. (F–I) WISH detected cmlc2 (F,F’,F”), vmhc (G,G’,G”), gata4 (H,H’,H”), and nppa (I,I’,I”) in embryos of the 48 hpf control, miR-183/96/182 mimics OE and miR-183/96/182 capped RNA OE (red arrow heads indicate separate cardiac primordia). (J) The mRNA expression levels of heart development-related genes in control, miR-183/96/182 mimics OE embryos were analyzed by RT-qPCR at 42 hpf. The number at the bottom right indicates the number of embryos. ** p < 0.01, *** p < 0.001, **** p <0.0001.