Figure 6

Co-expression of the miR-183 family leads to abnormal migration of endodermal cells. (A–C) Fluorescence image of the endoderm of 36 hpf Tg (sox17: eGFP) transgenic zebrafish embryo (ventral view). Control embryos show normal convergence of endoderm cells at the midline (A); Embryos of miR-183/96/182 mimics OE (B) and miR-183/96/182 capped RNA OE (C) both presented with holes in the central region of the endoderm tissue (indicated by white asterisks). (D,E) Quantification of endodermal defect phenotypes in miR-183/96/182 mimics OE (D) and miR-183/96/182 capped RNA OE (E) embryos. (F) The mRNA expression levels of the sox32 gene in the control, miR-183/96/182 mimics OE and miR-183/96/182 capped RNA OE embryos were analyzed by RT-qPCR at 36 hpf. (G–G”) WISH was used to detect the expression of sox32 in 9 hpf control (G), miR-183/96/182 mimics OE (G’), and miR-183/96/182 capped RNA OE (G”) embryos (ventral view; red arrow heads indicate the kupffer’s vesicle). The number at the bottom right indicates the number of embryos. *** p < 0.001.