FIGURE

Figure 7

ID

ZDB-FIG-250909-107

Publication

Contreras et al., 2025 - OpenEMMU: A versatile, open-source EdU multiplexing methodology for studying DNA replication and cell cycle dynamics

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Figure 7

DNA replication in self-organizing 3D human cardiac organoids

(A) Protocol outline to generate self-organizing and beating hiPSC-derived human cardiac organoids (hCO), showing two distinct imaging strategies: FFPE organoid confocal imaging (a’) and 3D light sheet microscopy (b’).

(B) FFPE-processed day 12 organoid and confocal tile imaging of EdU fluorescence in DNA replicating cells (n = 6). NKX2-5 and cTnT expressing cardiac progenitors are highlighted in the boxed area. NKX2-5 and EdU double-positive cells are also shown (∗, magnified box).

(C) Whole-mount light sheet microscopy (LSFM) 3D visualization of a hCO at day 19 showing EdU positive cells and cTnT expressing cells. DNA-labelled nuclei are also shown (n = 6).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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