FIGURE

Figure 3

ID

ZDB-FIG-250909-102

Publication

Contreras et al., 2025 - OpenEMMU: A versatile, open-source EdU multiplexing methodology for studying DNA replication and cell cycle dynamics

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Figure 3

Compatibility of OpenEMMU for multi-parametric analysis of DNA replication in mouse and human immune cells

(A) Outline of adult mice EdU injection and OpenEMMU protocol for studying DNA synthesis in various tissues and cells.

(B) Flow cytometry of EdU signal using isolated bone marrow cells (n = 3).

(C) Flow cytometry of EdU signal using isolated splenocytes, highlighting percentages of rare DNA replicating cells (CD11b+ and CD11c+) (n = 3).

(D) Outline of freshly isolated human peripheral blood mononuclear cells (PBMCs) evaluation for EdU incorporation and responses to various immunological stimuli.

(E) Gating strategy employed to detect DNA replication in activated human T-cells. The dashed lines indicate the selected gates.

(F) Cell stimulation with activating antibodies or exposure to infectious disease-relevant immune stimulants showing the quantification of EdU+ DNA replicating CD3⁺CD4⁺CD25⁺ cells (dashed lines) (n = 2).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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