ZFIN Image: Contreras et al., 2025, Figure 7

Image

Figure Caption

Figure 7

DNA replication in self-organizing 3D human cardiac organoids

(A) Protocol outline to generate self-organizing and beating hiPSC-derived human cardiac organoids (hCO), showing two distinct imaging strategies: FFPE organoid confocal imaging (a’) and 3D light sheet microscopy (b’).

(B) FFPE-processed day 12 organoid and confocal tile imaging of EdU fluorescence in DNA replicating cells (n = 6). NKX2-5 and cTnT expressing cardiac progenitors are highlighted in the boxed area. NKX2-5 and EdU double-positive cells are also shown (∗, magnified box).

(C) Whole-mount light sheet microscopy (LSFM) 3D visualization of a hCO at day 19 showing EdU positive cells and cTnT expressing cells. DNA-labelled nuclei are also shown (n = 6).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ iScience