FIGURE

Figure 8

ID

ZDB-FIG-250909-108

Publication

Contreras et al., 2025 - OpenEMMU: A versatile, open-source EdU multiplexing methodology for studying DNA replication and cell cycle dynamics

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Figure 8

Analysis of DNA replication in whole zebrafish larvae

(A) Danio rerio 5dpf larvae immersion protocol aimed at labeling DNA synthesis with EdU for 2h using FFPE (B) or whole-mount staining (D–F).

(B) FFPE-processed larva sagittal cross section and confocal tile imaging of EdU signal in DNA replicating cells (n = 3). αSMA-Cy3 immunolabeling depicts the developing musculature and growing myotomes. Insets (below) show the magnification of the boxed areas (a’-c’). Arrowhead shows a mitotic event.

(C and D) Depigmentation and DEEP-Clear protocol allow the whole-mount visualization of growing larvae (n = 6).

(E and F) EdU and DNA labeling revealing highly proliferative cells, visualized by tiling (E) and z stack (F) confocal microscopy.

(F) Insets (F) show the magnification of the boxed areas (a’-e’). CMZ, ciliary marginal zone; MTJ, myotendinous junction; nc, notochord; ms, myoseptum; mf, myofiber; bv, blood vessels.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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