Radiation-induced neuropathology in adult zebrafish brains. a Experimental timeline for whole-body irradiation with 20 Gy γ-rays (0.84 Gy/min, 24 min) followed by subsequent analyses. Examinations were conducted at 3, 7, and 14 dpi. These included brain histology, molecular assays, and behavioral tests using novel tank locomotor analysis. b Representative HE-stained images of adult zebrafish brains. The boxed areas show enlarged views of the optic tectum. c Quantification of inflammatory infiltrates in the brain parenchyma (n = 3 brains per group; one-way ANOVA). d Representative images of iNOS immunofluorescence and quantification of fluorescence intensity (n = 3 brain sections; one-way ANOVA). e Heatmap of neuroendocrine-related gene mRNA expression (esr2b, cyp19b, trβ, crhbp, crhr2) across time points. f–j Novel tank behavioral profiling: heatmaps depicting spatial distribution (top/bottom zones demarcated by the horizontal midline) (f); total distance (g); average velocity (h); number of transitions (i); latency to enter the top (j) (f–j: n = 6 zebrafish; one-way ANOVA). All the statistical data are presented as the means ± SEMs. Scale bars: 200 μm (b, top), 50 μm (b, boxed areas), 100 μm (d)
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