Fig. 7

In vitro therapeutic effects of Pep-Cu_5.4O@H151 nanodrugs on RIBI. a Representative images of intracellular ROS detection via the DCFH-DA fluorescent probe in BV2 cells. b Quantification of ROS fluorescence intensity (n = 3 fields; one-way ANOVA). c Representative images of mtROS detection via the MitoSOX fluorescent probe in BV2 cells. d Quantification of mtROS fluorescence intensity (n = 3 fields; one-way ANOVA). e Immunofluorescence images of M1-polarized macrophages stained for CD86 (Alexa Fluor 488, green). f Quantitative analysis of CD86 fluorescence intensity normalized to that of DAPI (n = 5 fields; one-way ANOVA). g Immunofluorescence images of M2-polarized macrophages stained for CD206 (Alexa Fluor 594, red). h Quantitative analysis of CD206 fluorescence intensity normalized to that of DAPI (n = 5 fields; one-way ANOVA). i SYBR Gold-enhanced superresolution imaging of live HT22 mitochondria in culture medium. j, k Mitochondrial morphology analysis of HT22 cells: mean area (j) and mean perimeter (k) (n = 6 mitochondria; Student’s t test). All the statistical data are presented as the means ± SEMs. Scale bars: 20 μm (a, c, e, and g), 10 μm (h)