FIGURE

Fig. 5

ID

ZDB-FIG-250724-42

Publication

Herdt et al., 2025 - In vivo measurement of an Apelin gradient with a genetically encoded APLNR conformation biosensor

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Fig. 5

APLNR-cpGFP biosensors visualize endogenous Aplnr activity in vivo.

a Representative confocal projection images of blood vessels in the trunk of triple transgenic Tg(fli1a:GAL4FF); Tg(UAS:RFP); Tg(UAS:APLNR(F233)-cpGFP) zebrafish embryos at 28 hours post-fertilization (hpf). b Quantification of APLNR(F233)-cpGFP delta fluorescence of ISVs compared to the DA/PCV. Each dot represents the mean of five analyzed ISVs per embryo or of the DA/PCV. c Representative confocal projection images of blood vessels in the trunk of triple transgenic Tg(fli1a:GAL4FF); Tg(UAS:RFP); Tg(UAS:APLNR(K235)-cpGFP) zebrafish embryos at 28 hpf. d Quantification of APLNR(K235)-cpGFP delta fluorescence intensity of ISVs compared to the DA/PCV. Each dot represents the mean of five analyzed ISVs per embryo or of the DA/PCV. e Representative confocal projection images of blood vessels in the trunk of triple transgenic Tg(fli1a:GAL4FF); Tg(UAS:RFP); Tg(UAS:APLNR(K235)-cpGFP) zebrafish embryos injected with apln, apela CRISPANTs at 28 hpf. f Quantification of APLNR(K235)-cpGFP delta fluorescence of ISVs in apln, apela CRISPANTs compared to control siblings. Each dot represents the mean of up to five analyzed ISVs per embryo. g Representative confocal projection images of blood vessels in the trunk of Tg(fli1a:GAL4FF); Tg(UAS:RFP); Tg(UAS:APLNR(K235)-cpGFP); Tg(hsp70l:apln) zebrafish embryos at 28 hpf. h Quantification of APLNR(K235)-cpGFP delta fluorescence of ISVs in Apelin ligand overexpression Tg(hsp70l:apln) embryos compared to control wildtype siblings. Each dot represents the mean of five analyzed ISVs per embryo. Data are presented as mean values ± SEM. (N number of embryos, n either ISVs or DA/PCV: b DA/PCV N/n: 15/15, ISV N/n: 15/75; d DA/PCV N/n: 16/16, ISV N/n: 16/80; f Control: DA/PCV N/n: 13/13, ISV N/n: 13/65; apln, apela CRISPANTs: DA/PCV N/n: 9/9, ISV N/n: 9/38; h wildtype siblings: DA/PCV N/n: 11/11, ISV N/n: 11/55; Tg(hsp70l:apln): DA/PCV N/n: 17/17, ISV N/n: 17/85). Statistical analysis was performed by using a two-tailed unpaired Student’s t-test with Welch’s correction. Scale bars 15 µm. cpGFP circularly permuted GFP, ISV intersegmental vessel, DA dorsal aorta, PCV posterior cardinal vein.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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