FIGURE 8

Transcriptional regulation of her3 by Pou5f3 and SoxB1 in cultured cells. A, B. Effects of Pou5f3 and Sox3 (A) or Pou5f3 and Sox2 (B) on the expression of pHer3[−4. 0]‐Luc in HEK293T (left) or P19 (right) cells. The expression levels of the reporter in the presence of the effectors indicated on the abscissa are shown relative to the control level when egfp was used as an effector. C. Effects of Pou5f3 and Sox3 on the expression of luciferase constructs lacking NCR1, NCR2, or both. The expression levels of the reporter genes in HEK293T cells in the presence of the expression plasmids for egfp (blue), pou5f3 (magenta), sox3 (gray), and pou5f3 plus sox3 (green) are shown relative to the luciferase expression for pGL4 in the presence of egfp. The expression levels of firefly luciferase were standardized by Renilla luciferase expression as an internal control. The experiments were repeated three times, resulting in essentially the same results. Error bars, standard deviations of means. *, p < 0.05; **, p < 0.01; ***, p < 0.001.