Figure 4

Haploinsufficiency of Kdm6a has skewed myelopoiesis in embryonic and adult zebrafish. A,B) WISH for mfap4 A) and %phenotype distribution B) in embryos at 3 dpf as indicated (n = 4, mean ± SD, Student's t test). C,D) WISH for lyz C) and %phenotype distribution D) in embryos at 3 dpf as indicated (n  = 4, mean ± SD, Student's t test). E,F) WISH for hbae1 E) and %phenotype distribution F) in embryos at 3 dpf as indicated (n = 4, mean ± SD, Student's t test). G–L) qPCR analysis of mfap4, mpeg1, lyz, mpx, hbae1, and alas2 expression in WT control and kdm6a^+/− mutant embryos at 3 dpf (n = 3, mean ± SD, Student's t test). M) qPCR analysis of lineage differentiation‐related transcription factors expression including cebpd, cebpg, cebpz, irf8, gata1a, and ccr9a in cd41:eGFP^low HSPCs isolated from WT control or kdm6a^+/− mutant embryos (n = 3, mean ± SD, one‐way ANOVA). N‐N’) Hematoxylin and eosin staining of paraffin‐embedded sections of kidney from representative WT control or kdm6a^+/− mutant adults. O‐O’) Hematoxylin and eosin staining of paraffin‐embedded sections of spleen from representative WT control or kdm6a^+/− mutant adults. P‐P’) May–Grünwald–Giemsa staining of whole kidney marrow (KM) cells presentative 3 kdm6a^+/− fish with CMML‐like phenotypes (totally 12 kdm6a^+/− adults were used for experiment). *p <0.05; **p <0.01; ***p < 0.001.