Figure 3

Kdm6a regulates HSPC production via Syk‐associated inflammatory response in a H3K27me3‐dependent manner. A) Heatmap showing differential expression of inflammatory‐associated genes. B) Enrichment plot of the TNFα signaling via NF‐κB between differentially regulated genes in kdm6a morphants and their WT controls by GSEA. C,D) WISH for runx1 C) and %phenotype distribution D) in WT control, kdm6a mutants, and kdm6a mutants with ikbaa morphants at 28 hpf as indicated (n = 4, mean ± SD, one‐way ANOVA). E) qPCR analysis of syk expression in sorted endothelial cells from WT control and kdm6a mutants at 28 hpf (n = 3, mean ± SD, Student's t test). F,G) WISH for runx1 F) and %phenotype distribution G) in WT control, kdm6a mutants, and kdm6a mutants with kdrl:syk constructs at 28 hpf (n = 4, mean ± SD, one‐way ANOVA). H,I) WISH for runx1 H) and %phenotype distribution I) in WT control, kdm6a mutants, kdm6a mutants with kdm6a mRNA, and kdm6a mutants with kdm6a^H1134A mRNA at 28 hpf (n = 4, mean ± SD, one‐way ANOVA). J) ChIP‐qPCR analyses of syk promoter in kdm6a morphants and their WT controls by using an anti‐H3K27me3 antibody at 28 hpf (n = 3, mean ± SD, Student's t test). *p <0.05; **p <0.01; ***p < 0.001.